應用
Y(II)或ΔF/Fm’ 或 (Fm’ – Fs )/Fm’) 是經(jīng)受時(shí)間考驗的光適應測量參數，比Fv/Fm對更多類(lèi)型的植物脅迫更加敏感。已有的大量證據表明Fv/Fm對許多種植物脅迫和健康植物的光系統II的測量十分出色，而Y(II)或光量子產(chǎn)額則可測量實(shí)際光照下光適應環(huán)境和生理狀況的光系統II的效率。
原理
采用調制飽和脈沖原理，測量植物的葉綠素熒光，測量參數包括植物的光量子產(chǎn)額Y(II)及相對電子傳遞速率ETR，最大光化學(xué)效率Fv/Fm，同時(shí)還可測量PAR、葉溫、相對濕度和葉片吸光率等環(huán)境參數。
特點(diǎn)
葉片吸光率測量：提供葉片吸收測量及隨環(huán)境變化導致的葉片吸收變化。根據Eichelman (2004) 葉片吸收在健康植物的變化范圍在0.7~0.9 之間。因此，為獲得準確的ETR或“J”，Y(II)測量?jì)x提供了一個(gè)可靠的測量方法，
Fv/Fm測量單元：用于暗適應測量。
先進(jìn)的PAR葉夾：采用底部葉夾打開(kāi)裝置，防止測量時(shí)誤操作打開(kāi)葉夾。對傳感器進(jìn)行余弦校正，確保葉片相對測量光的角度不變。
Fm’校正：對于具有高光照強度歷史的植物，*關(guān)閉光反應中心是一個(gè)問(wèn)題，Y(II)測量?jì)x使用Loriaux &Genty 2013的方法進(jìn)行Fm’ 校正，確?？梢詼y得準確的Fm’ 。
自動(dòng)調制光設定：快速準確自動(dòng)的調整合適的調制光強，避免人工操作的誤差。
先進(jìn)算法避免飽和脈沖NPQ：采用25ms內8點(diǎn)的平均值確定Fm、Fm’、Fo、Fs，消除飽和脈沖NPQ的影響和電子噪音。
更精確的葉溫測量：采用非接觸式紅外測量，測量精度可達±0.5℃。
直接測量相對濕度：含有測量氣體交換使用的固態(tài)傳感器，可測量相對濕度。
降低葉片遮擋的設計：傾斜的角度減少對葉片的遮擋，可以測量擬南芥等小葉。
系統組成
標配： Y(II)光量子產(chǎn)額測量?jì)x，Fv/Fm測量?jì)x及10個(gè)暗適應葉夾，2個(gè)電池，2個(gè)充電器，一個(gè)便攜箱，文件U盤(pán)。
技術(shù)指標
測量參數：
Y(II)或ΔF/Fm‘、ETR、PAR、Tleaf、相對濕度、Fms或Fm’、Fs、α(葉片吸收率)、FV/FM、FV/FO，FO, FM, FV。
監測模式：允許長(cháng)時(shí)間監測
技術(shù)參數：
Y(II): 光適應測量, 穩態(tài)光合作用下的環(huán)境光
光源
飽和脈沖： LED白光源，使用PAR葉夾時(shí)可達7000μmols
調制光：紅光，LED 660nm，具有690nm窄通過(guò)濾器。
光化光源：環(huán)境光
檢測方法：脈沖調制法
PAR：測量400-700nm，余弦校正 ±2umols
Fv/Fm：暗適應測量
光源：LED紅光飽和光閃，可達6000umols；
調制光：660nmLED 紅光，690nm濾波器
調制光可以根據實(shí)際測量自動(dòng)調節到合適的強度，減少手動(dòng)調節誤差，
相對濕度：0%~100%，±2%。
檢測器&過(guò)濾器：具有700~750nm帶通過(guò)濾的PIN光電二極管
可選配三腳架。
顯示：132 X 30 pixel 液晶顯示屏
取樣速率：1~10000點(diǎn)/秒自動(dòng)切換。
測量時(shí)間：最短3s或也可設置長(cháng)期監測模式
存儲空間：2GB
輸出：USB下載數據，用Excel查看，無(wú)需安裝其他專(zhuān)用軟件
供電：USB鋰離子電池(普通充電寶)，可用8小時(shí)
尺寸：便攜箱尺寸為14”x 11”x 6”，儀器為9’’長(cháng)
質(zhì)量：Y(II) 測量?jì)x0.45 kg
Fv/Fm測量?jì)x0.36 kg.
加便攜箱和附件總重1.95 kg.
工作溫度：0℃ ~ 50℃
產(chǎn)地
美國
文獻
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